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Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis in 293 cell line lysates (35ug/lane).

COBRA1 Antibody

CATALOG NUMBER: 57-973

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This COBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human COBRA1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NELFB
Additional Names:
Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182
Protein Accession Number:
Q8WX92
Protein Gi Number:
38372378
Ncbi Gene Id Number:
25920
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NELFB is a subunit of negative elongation factor (NELF),which also includes NELFA (WHSC2; MIM 606026), either NELFC orNELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF actswith DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4(SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to causetranscriptional pausing of RNA polymerase II (see MIM 180660)(Narita et al., 2003 [PubMed 12612062]).
Background Reference 1:
Sun, J., et al. J. Cell. Biochem. 103(6):1798-1807(2008)
Background Reference 2:
Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007) :
Background Reference 3:
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Background Reference 4:
Narita, T., et al. Mol. Cell 26(3):349-365(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-973

List Size:
400 ul

List Price:

$495.00

Datasheet
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