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Western blot analysis of lysed extracts from Mouse heart tissue using cofilin1/cofilin2 (phospho-Tyr88).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin1/cofilin2 (Phospho-Tyr88).
Western blot analysis of lysed extracts from Mouse heart tissue using cofilin1/cofilin2 (phospho-Tyr88).

cofilin1 / cofilin2 (phospho Tyr88) Antibody

CATALOG NUMBER: 79-240

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
cofilin1/cofilin2 (phospho-Tyr88) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y (p) -E-T) derived from Human coflin1/cofilin2.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of cofilin1/cofilin2 only when phosphorylated at tyrosine 88.
Predicted Molecular Weight:
19 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CFL1 CFL2
Additional Names:
CFL, HEL-S-15, ESR, NR3A1, CFL1/CFL2
Protein Accession Number:
NP_005498.1;NP_068733.1
Protein Gi Number:
5031635 / 14719392
Ncbi Gene Id Number:
1072;1073
Background:
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Background Reference 1:
Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830. Arber, S. et al. (1998) Nature 393, 805-809. Yang, N. et al. (1998) Nature 393, 809-812.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-240

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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