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46-962 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-962 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

COG1 Antibody

CATALOG NUMBER: 46-962

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KAKSTRNIETKAQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 110kDa band observed in Human Brain (Cerebellum, Amygdala) lysates (calculated MW of 109kDa according to NP_061184.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
COG1
Additional Names:
COG1, component of oligomeric golgi complex 1, CDG2G, DKFZp762L1710, KIAA1381, LDLB, conserved oligomeric Golgi complex protein 1, low density lipoprotein receptor defect B complementing
Protein Accession Number:
NP_061184.1
Protein Gi Number:
21237783
Ncbi Gene Id Number:
9382
Background Reference 1:
Vasile E, Oka T, Ericsson M, Nakamura N, Krieger M. IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex. Exp Cell Res. 2006 Oct 1;312(16):3132-41.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-962

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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