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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
COG2 Antibody (RB17913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

COG2 Antibody

CATALOG NUMBER: 63-918

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This COG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-124 amino acids from the N-terminal region of human COG2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COG2
Additional Names:
Conserved oligomeric Golgi complex subunit 2, COG complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein, COG2, LDLC
Protein Accession Number:
Q14746
Protein Gi Number:
2498512
Ncbi Gene Id Number:
22796
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
COG2 encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi complex. The encoded protein specifically interacts with the USO1 vesicle docking protein and may be necessary for normal Golgi ribbon formation and trafficking of Golgi enzymes.
Background Reference 1:
Edwards, T.L., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (5), 721-735 (2009)
Background Reference 2:
Luke, M.M., et al. Stroke 40(2):363-368(2009)
Background Reference 3:
Shiffman, D., et al. Arterioscler. Thromb. Vasc. Biol. 28(1):173-179(2008)
Background Reference 4:
Sohda, M., et al. Traffic 8(3):270-284(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-918

List Size:
400 ul

List Price:

$495.00

Datasheet
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