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Western blot analysis of COH1 in SK-N-SH cell lysate with COH1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of COH1 in human brain tissue with COH1 antibody at 20 μg/mL.
Western blot analysis of COH1 in SK-N-SH cell lysate with COH1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

COH1 Antibody

CATALOG NUMBER: 6931

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
COH1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human COH1.
The immunogen is located within amino acids 90 - 140 of COH1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
COH1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
COH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
COH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS13B
Additional Names:
COH1 Antibody: CHS1, COH1, CHS1, KIAA0532, Vacuolar protein sorting-associated protein 13B, Cohen syndrome protein 1
Protein Accession Number:
NP_056058
Protein Gi Number:
35493725
Ncbi Gene Id Number:
157680
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
COH1 Antibody: COH1 (Cohen syndrome protein 1), also known as VPS13B (vacuolar protein sorting-associated protein 13B) or CHS1, belongs to the VPS13 family and may function in vesicle-mediated transport and sorting of proteins within the cell. COH1 is widely expressed and multiple alternatively spliced transcript variants have been observed. Mutations in this gene have been associated with Cohen syndrome. COH1 is a Golgi-localized peripheral membrane protein and plays a critical role in Golgi (re)assembly.
Background Reference 1:
Velayos-Baeza A, Vettori A, Copley RR, et al. Analysis of the human VPS13 gene family. Genomics 2004; 84:536-49.
Background Reference 2:
Kolehmainen J, Black GC, Saarinen A, et al. Cohen syndrome is caused by mutations in a novel gene, COH1, encoding a transmembrane protein with a presumed role in vesicle-mediated sorting and intracellular protein transport. Am. J. Hum. Genet. 2003; 72:1359-69.
Background Reference 3:
Seifert W, Kühnisch J, Maritzen T, et al. Cohen syndrome-associated protein, COH1, is a novel, giant Golgi matrix protein required for Golgi integrity. J. Biol. Chem. 2011; 286:37665-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6931

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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