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Western blot analysis of various lysates, using COL11A1 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.
Confocal imaging of A-204 cells using COL11A1 Rabbit mAb(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Western blot analysis of various lysates, using COL11A1 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.

COL11A1 Rabbit Recombinant Antibody (82-949)

CATALOG NUMBER: 82-949

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:2000 - 1:12000, IF/ICC,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
A-204
Predicted Molecular Weight:
167kDa/176kDa/181kDa/182kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
COL11A1
Additional Names:
STL2; COLL6; CO11A1; DFNA37; COL11A1
Protein Accession Number:
P12107
Ncbi Gene Id Number:
1301
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-949

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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