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Western blot analysis of COL18A1 Antibody in mouse lung tissue lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of COL18A1 Antibody in mouse lung tissue lysates (35ug/lane)

COL18A1 Antibody

CATALOG NUMBER: 63-671

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This COL18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-263 amino acids from the N-terminal region of human COL18A1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
178 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COL18A1
Additional Names:
Collagen alpha-1(XVIII) chain, Endostatin, COL18A1
Protein Accession Number:
P39060
Protein Gi Number:
215274264
Ncbi Gene Id Number:
80781
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
COL18A1 is encoding the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein.
Background Reference 1:
Fu,Y.,et.al., Biochemistry 48 (49), 11655-11663 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-671

List Size:
400 ul

List Price:

$495.00

Datasheet
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