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Western Blot at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.

COL1A2 Antibody

CATALOG NUMBER: 55-916

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This COL1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human COL1A2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
129 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COL1A2
Additional Names:
Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2
Protein Accession Number:
P08123
Protein Gi Number:
296439507
Ncbi Gene Id Number:
1278
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the pro-alpha2 chain of type I collagenwhose triple helix comprises two alpha1 chains and one alpha2chain. Type I is a fibril-forming collagen found in most connectivetissues and is abundant in bone, cornea, dermis and tendon.Mutations in this gene are associated with osteogenesis imperfectatypes I-IV, Ehlers-Danlos syndrome type VIIB, recessiveEhlers-Danlos syndrome Classical type, idiopathic osteoporosis, andatypical Marfan syndrome. Symptoms associated with mutations inthis gene, however, tend to be less severe than mutations in thegene for the alpha1 chain of type I collagen (COL1A1) reflectingthe different role of alpha2 chains in matrix integrity. Threetranscripts, resulting from the use of alternate polyadenylationsignals, have been identified for this gene. [provided by R.Dalgleish].
Background Reference 1:
Blades, H.Z., et al. Bone 47(5):989-994(2010)
Background Reference 2:
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Background Reference 3:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 4:
Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-916

List Size:
400 ul

List Price:

$495.00

Datasheet
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