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Western blot analysis in HepG2 cell line lysates (35ug/lane).
COL21A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of COL21A1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

COL21A1 Antibody

CATALOG NUMBER: 56-246

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This COL21A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the N-terminal region of human COL21A1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
99 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COL21A1
Additional Names:
Collagen alpha-1(XXI) chain, COL21A1, COL1AL
Protein Accession Number:
Q96P44
Protein Gi Number:
74752071
Ncbi Gene Id Number:
81578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the alpha chain of type XXI collagen, amember of the FACIT collagen family (fibril-associated collagenswith interrupted helices). Type XXI collagen is localized totissues containing type I collagen so, like other members of thiscollagen family, it may serve to maintain the integrity of theextracellular matrix. An alternatively spliced transcript varianthas been described, but its full-length nature has yet to bedetermined.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Chou, M.Y., et al. Genomics 79(3):395-401(2002)
Background Reference 3:
Tuckwell, D. Matrix Biol. 21(1):63-66(2002)
Background Reference 4:
Fitzgerald, J., et al. FEBS Lett. 505(2):275-280(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-246

List Size:
400 ul

List Price:

$495.00

Datasheet
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