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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent image of U251 cells stained with COL4A1 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate(from left to right), using COL4A1 Antibody at 1:1000 at each lane.
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.

COL4A1 Antibody

CATALOG NUMBER: 62-860

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This COL4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human COL4A1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:25
Predicted Molecular Weight:
161 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COL4A1
Additional Names:
Collagen alpha-1(IV) chain, Arresten, COL4A1
Protein Accession Number:
P02462
Protein Gi Number:
125987809
Ncbi Gene Id Number:
1282
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
COL4A1 is the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Background Reference 1:
de Vries,L.S., Ann. Neurol. 65 (1), 12-18 (2009)
Background Reference 2:
Mazouni,C., Br. J. Cancer 99 (1), 68-71 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-860

List Size:
400 ul

List Price:

$519.00

Datasheet
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