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45-412 (0.5ug/ml) staining of Mouse Heart lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-412 (0.5ug/ml) staining of Mouse Heart lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

COL4A3BP Antibody

CATALOG NUMBER: 45-412

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: DHDSAPLNNRC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60-65kDa band observed in Mouse Heart lysates (calculated MW of 71.1kDa according to Mouse NP_075909.1). An additional band of unknown identity was also consistently observed at 25kDa. Both bands were successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1ug/ml. Peimary incubation 1 hour at room temperature.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1)
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
COL4A3BP
Additional Names:
COL4A3BP, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein , CERT, CERTL, FLJ20597, GPBP , STARD11, Goodpasture antigen-binding protein, START domain containing 11, alpha 3 type IV collagen binding protein, ceramide transporter,
Protein Accession Number:
NP_005704.1,NP_112729.1
Protein Gi Number:
5031717
Ncbi Gene Id Number:
10087
Background Reference 1:
Swanton C, Marani M, Pardo O, Warne PH, Kelly G, Sahai E, Elustondo F, Chang J, Temple J, Ahmed AA, Brenton JD, Downward J, Nicke B. Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs. Cancer. 2007 Jun;11(6):498-512.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-412

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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