COL4A6 Antibody

CATALOG NUMBER: 42-181

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-PSPEFETETLHNKES
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
COL4A6
Additional Names:
COL4A6, collagen, type IV, alpha 6, MGC88184, OTTHUMP00000023834, OTTHUMP00000023835, collagen IV, alpha-6 polypeptide, collagen alpha 6 type IV, collagen of basement membrane, alpha-6, dJ889N15.4 (Collagen Alpha 6(IV)), type IV alpha 6 collagen
Protein Accession Number:
NP_001838.2,NP_378667.1
Protein Gi Number:
148536823
Ncbi Gene Id Number:
1288
Background Reference 1:
Zhang X, Zhou J, Reeders ST, Tryggvason K Structure of the human type IV collagen COL4A6 gene, which is mutated in Alport syndrome-associated leiomyomatosis Genomics. 1996 May 33 (3): 473-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-181

List Size:
0.1 mg

List Price:

$468.00

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