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Western blot analysis in U251 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
COL5A1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in U251 cell line lysates (35ug/lane).

COL5A1 Antibody

CATALOG NUMBER: 55-212

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
184 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COL5A1
Additional Names:
Collagen alpha-1(V) chain, COL5A1
Protein Accession Number:
P20908
Protein Gi Number:
85687376
Ncbi Gene Id Number:
1289
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
COL5A1 encodes an alpha chain for one of the lowabundance fibrillar collagens. Fibrillar collagen molecules aretrimers that can be composed of one or more types of alpha chains.Type V collagen is found in tissues containing type I collagen andappears to regulate the assembly of heterotypic fibers composed ofboth type I and type V collagen. COL5A1 product is closelyrelated to type XI collagen and it is possible that the collagenchains of types V and XI constitute a single collagen type withtissue-specific chain combinations. Mutations in this gene areassociated with Ehlers-Danlos syndrome, types I and II. [providedby RefSeq].
Background Reference 1:
Vitart, V., et al. Hum. Mol. Genet. 19(21):4304-4311(2010)
Background Reference 2:
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Background Reference 3:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 4:
Posthumus, M., et al. Med Sci Sports Exerc (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-212

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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