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Western blot analysis in CEM cell line lysates (35ug/lane).
COL8A2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of COL8A2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in CEM cell line lysates (35ug/lane).

COL8A2 Antibody

CATALOG NUMBER: 56-083

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COL8A2
Additional Names:
Collagen alpha-2(VIII) chain, Endothelial collagen, COL8A2
Protein Accession Number:
P25067
Protein Gi Number:
45644957
Ncbi Gene Id Number:
1296
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the alpha 2 chain of type VIII collagen.The protein is a major component of the basement membrane of thecorneal endothelium and forms homo- or heterotrimers with alpha 1(VIII) type collagens. Defects in this gene are associated withFuchs endothelial corneal dystrophy and posterior polymorphouscorneal dystrophy type 2.
Background Reference 1:
Hemadevi, B., et al. BMC Ophthalmol 10,(2010) :
Background Reference 2:
Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009)
Background Reference 3:
Liskova, P., et al. BrOphthalmol 91(12):1717-1718(2007)
Background Reference 4:
Aldave, A.J., et al. Cornea 26(8):963-965(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-083

List Size:
400 ul

List Price:

$495.00

Datasheet
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