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45-413 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
45-413 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Collagen Antibody

CATALOG NUMBER: 45-413

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: QKELECEGGQRERPQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise all four reported isoforms (NP_542411.2; NP_542412.2; NP_542410.2; NP_001157243.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
COL11A2
Additional Names:
COL11A2, collagen, type XI, alpha 2, DAQB-79P13.8, HGNC:2187, DFNA13, HKE5, PARP, STL3, Collagen alpha 2(XI)
Protein Accession Number:
NP_542411.2,NP_542412.2,NP_542410.2,NP_001157243.1
Protein Gi Number:
18201917
Ncbi Gene Id Number:
1302
Background Reference 1:
Harel T, Rabinowitz R, Hendler N, Galil A, Flusser H, Chemke J, Gradstein L, Lifshitz T, Ofir R, Elbedour K, Birk OS. COL11A2 mutation associated with autosomal recessive Weissenbacher-Zweymuller syndrome: molecular and clinical overlap with otospondylomegaepiphyseal dysplasia (OSMED). Am J Med Genet A. 2005 Jan 1;132(1):33-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-413

List Size:
0.1 mg

List Price:

$430.00

Out of stock

Datasheet
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