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Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GJA4 antibody detected the GJA4 protein (arrow).
Connexin 37 Antibody (GJA4) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Connexin 37 Antibody (GJA4) for immunohistochemistry.
Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GJA4 antibody detected the GJA4 protein (arrow).

Connexin 37 Antibody

CATALOG NUMBER: 58-178

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This Connexin 37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the C-terminal region of human Connexin 37.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GJA4
Additional Names:
Gap junction alpha-4 protein, Connexin-37, Cx37, GJA4
Protein Accession Number:
P35212
Protein Gi Number:
8928555
Ncbi Gene Id Number:
2701
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gap junctions permit direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular communication. Gap junction channels consist of connexin protein subunits encoded by a multigene family. Erythrokeratodermia variabilis (EKV) is an autosomal dominant disorder of keratinization characterized by migratory erythematous lesions and fixed keratotic plaques. Mutations in the GJB3 gene have been reported in some but not all families, although it has been postulated that the absence of connexin 30.3 can be compensated by other connexins.
Background Reference 1:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Background Reference 2:
Saito, T., et al., Int. J. Cancer 86(1):67-70 (2000).
Background Reference 3:
Boerma, M., et al., J. Intern. Med. 246(2):211-218 (1999).
Background Reference 4:
Krutovskikh, V., et al., Carcinogenesis 17(8):1761-1763 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-178

List Size:
400 ul

List Price:

$495.00

Datasheet
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