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Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121) at 1:1000 at each lane.
(LEFT)Western blot analysis of anti-GJA1 Antibody (N-term) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of hGJA1-N121.Connexin in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121) at 1:1000 at each lane.

Connexin 43 Antibody

CATALOG NUMBER: 58-174

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine
Immunogen:
This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GJA1
Additional Names:
Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL
Protein Accession Number:
P17302
Protein Gi Number:
117706
Ncbi Gene Id Number:
2697
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Background Reference 1:
Spinella, F., et al., J. Biol. Chem. 278(42):41294-41301 (2003). Contreras, J.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100(20):11388-11393 (2003). Qin, H., et al., J. Biol. Chem. 278(32):30005-30014 (2003). Cameron, S.J., et al., J. Biol. Chem. 278(20):18682-18688 (2003). Ma, X.D., et al., World J. Gastroenterol. 9(5):946-950 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-174

List Size:
400 ul

List Price:

$519.00

Datasheet
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