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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.

Connexin Antibody

CATALOG NUMBER: 50-200

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Chicken, Dog, Frog, Human, Mouse, Rat, Sheep, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Chicken, Dog, Frog, Human, Mouse, Rat, Sheep, Zebrafish
Immunogen:
Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Predicted Molecular Weight:
43
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
Connexin antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Gja1
Protein Accession Number:
P08050
Protein Gi Number:
117708
Ncbi Gene Id Number:
24392
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Background Reference 1:
Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
Background Reference 2:
Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
Background Reference 3:
Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-200

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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