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43-466 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-466 (5ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, DAB-staining.
43-466 (1ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-466 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-466 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

contactin 1 Antibody

CATALOG NUMBER: 43-466

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-QVTSQEYSARLEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in Mouse Brain, and approx 150kDa observed in Human and Rat Brain lysates (calculated MW of 113kDa according to according to Human, NP_001834.2, Mouse NP_031753.1 and Rat NP_476459.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Mouse Brain (Cerebral Cortex). Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_001834.2) and isoform 2 (NP_778203.1).
Predicted Molecular Weight:
Approx 150kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
contactin 1
Additional Names:
CNTN1, contactin 1, F3, GP135, contactin-1, glycoprotein gP135, neural cell surface protein F3
Protein Accession Number:
NP_001834.2,NP_778203.1
Protein Gi Number:
28373117
Ncbi Gene Id Number:
1272
Background Reference 1:
Lamprianou S, Chatzopoulou E, Thomas JL, Bouyain S, Harroch S.A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-466

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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