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43-191 (0.5ug/ml) staining of Mouse Olfactory bulb (lanes1 and 3) and Cerebral cortex (lanes 2 and 4), comparing wildtype (lanes 3 and 4) with KO mice (lanes 1 and 2). The last lane contains a lysate of HEK293 overexpressing Mouse Cntn4 (lysate (35ug pro
43-191 (0.5ug/ml) staining of Mouse Olfactory bulb (lanes1 and 3) and Cerebral cortex (lanes 2 and 4), comparing wildtype (lanes 3 and 4) with KO mice (lanes 1 and 2). The last lane contains a lysate of HEK293 overexpressing Mouse Cntn4 (lysate (35ug pro

Contactin 4 / Big-2 Antibody

CATALOG NUMBER: 43-191

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RRADGKPIARK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Brain lysates and not in the same lysates from the KO mice (calculated MW of 113kDa according to NP_001103219.1). Recommended concentration: 0.5-1.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001103219.1; NP_766592.2). Reported variants represent identical protein: NP_766592.2, NP_001103221.1
Predicted Molecular Weight:
Approx 150kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Contactin 4 / Big-2
Additional Names:
Cntn4, contactin 4, 9630050B05, Axcam, BIG-2, axonal-associated cell adhesion molecule, brain-derived immunoglobulin superfamily molecule, brain-derived immunoglobulin superfamily protein 2, contactin-4
Protein Accession Number:
NP_001103219.1,NP_766592.2
Protein Gi Number:
157909807
Ncbi Gene Id Number:
152330
Background Reference 1:
Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y.BIG-2 mediates olfactory axon convergence to target glomeruli.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-191

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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