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43-187 (0.5ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
43-187 (0.5ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

COPA Antibody

CATALOG NUMBER: 43-187

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: CTRASNLENST
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 140kDa band observed in Human Prostate lysates (calculated MW of 138kDa according to NP_001091868.1and NP_004362.2). Recommended concentration: 0.5-2ug/ml.An additional band of 52kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001091868.1; NP_004362.2).
Predicted Molecular Weight:
Approx 140kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
COPA
Additional Names:
COPA, coatomer protein complex, subunit alpha, FLJ26320, HEP-COP, alpha coat protein, xenin
Protein Accession Number:
NP_001091868.1,NP_004362.2
Protein Gi Number:
148536853
Ncbi Gene Id Number:
1314
Background Reference 1:
Kano F, Yamauchi S, Yoshida Y, Watanabe-Takahashi M, Nishikawa K, Nakamura N, Murata M, Yip1A regulates the COPI-independent retrograde transport from the Golgi complex to the ER.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-187

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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