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Western Blot at 1:1000 dilution Lane 1: A375 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
COPE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of COPE Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution Lane 1: A375 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

COPE Antibody

CATALOG NUMBER: 55-787

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Hamster, Mouse
Immunogen:
This COPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-300 amino acids from the C-terminal region of human COPE.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COPE
Additional Names:
Coatomer subunit epsilon, Epsilon-coat protein, Epsilon-COP, COPE
Protein Accession Number:
O14579
Ncbi Gene Id Number:
11316
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is an epsilon subunit of coatomerprotein complex. Coatomer is a cytosolic protein complex that bindsto dilysine motifs and reversibly associates with Golginon-clathrin-coated vesicles. It is required for budding from Golgimembranes, and is essential for the retrograde Golgi-to-ERtransport of dilysine-tagged proteins. Coatomer complex consists ofat least the alpha, beta, beta', gamma, delta, epsilon and zetasubunits. Alternatively spliced transcript variants encodingdifferent isoforms have been identified.
Background Reference 1:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 2:
Morikawa, R.K., et al. J. Biol. Chem. 284(39):26620-26630(2009)
Background Reference 3:
Maruyama, S., et al. Mol. Cell. Biochem. 307 (1-2), 73-82 (2008) :
Background Reference 4:
Goryachev, A.B., et al. PLoS Comput. Biol.(12), E172 (2006) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-787

List Size:
400 ul

List Price:

$495.00

Datasheet
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