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Western blot analysis of extracts of various cell lines, using COQ7 antibody (23-170) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse lung using COQ7 antibody (23-170) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COQ7 antibody (23-170) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using COQ7 antibody (23-170) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using COQ7 antibody (23-170) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using COQ7 antibody (23-170) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.

COQ7 Antibody

CATALOG NUMBER: 23-170

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 37-217 of human COQ7 (NP_057222.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse skeletal muscle
Positive Control 2:
Mouse brain
Predicted Molecular Weight:
Observed: 26kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
COQ7
Additional Names:
Ubiquinone biosynthesis protein COQ7 homolog, Coenzyme Q biosynthesis protein 7 homolog, Timing protein clk-1 homolog, COQ7
Ncbi Gene Id Number:
10229
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is similar to a mitochondrial di-iron containing hydroxylase in Saccharomyces cerevisiae that is involved with ubiquinone biosynthesis. Mutations in the yeast gene lead to slower development and longer life span. Alternatively spliced transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-170

List Size:
100 uL

List Price:

$436.00

Datasheet
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