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HEK293 overexpressing CORO1A and probed with 45-421 (mock transfection in first lane).
45-421 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-421 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-421 (0.3ug/ml) staining of Human Lymphoblastoid (RIPA buffer, (35ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing CORO1A and probed with 45-421 (mock transfection in first lane).

Coronin 1 Antibody

CATALOG NUMBER: 45-421

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KRLDRLEETVQAK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Lymphoblastoid and Human T cell line MOLT4 lysates (calculated MW of 51.0kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CORO1A
Additional Names:
CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPINA, Clabp TACO, coronin, actin binding protein, 1A, coronin-1, coronin, actin-binding, 1A, coronin, actin-binding protein, 1A, FLJ41407, MGC117380,
Protein Accession Number:
NP_009005.1
Protein Gi Number:
5902134
Ncbi Gene Id Number:
11151
Background Reference 1:
Ferrari G, Langen H, Naito M, Pieters J. A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell. 1999 May 14;97(4):435-47.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-421

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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