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45-044 (0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-044 (0.1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
45-044 (0.03ug/ml) staining of HEK293 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
45-044 (0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cortactin Antibody

CATALOG NUMBER: 45-044

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: WKASAGHAVSIA-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in Human, Mouse and Rat Brain lysates and in lysates of cell line HEK293 (calculated MW of 61.6kDa according to Human NP_005222.2 and of 61.2kDa according to Mouse NP_031829.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paradis et al, J Cell Sci. 2008 Jun 15;121(Pt 12):1965-72; PMID 18495841). Recommended concentration: 0.01-0.03ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CTTN
Additional Names:
EMS1, CORTACTIN, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), Cttn, oncogene EMS1, CTTN, FLJ34459, 1110020L01Rik
Protein Accession Number:
NP_005222.2,NP_612632.1
Protein Gi Number:
20357552
Ncbi Gene Id Number:
2017
Background Reference 1:
Schuuring E, Verhoeven E, Mooi WJ, Michalides RJ. Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 1992 Feb;7(2):355-61

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-044

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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