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Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using Cortactin (Phospho-Tyr466).
Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using Cortactin (Phospho-Tyr466).

Cortactin (phospho Tyr466) Antibody

CATALOG NUMBER: 79-489

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Cortactin (Phospho-Tyr466) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y (p) -E-T) derived from Human CORTACTIN.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.
Predicted Molecular Weight:
85 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CTTN
Additional Names:
EMS1, PKB, RAC, Amplaxin, CTTN, EMS1, SRC8
Protein Accession Number:
NP_005222.2
Protein Gi Number:
20357552
Ncbi Gene Id Number:
2017
Background:
Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
Background Reference 1:
Luo C, et al. (2006) J Biol Chem ; 281 (40): 30081-30093Head JA, et al. (2003) Mol Biol Cell ;14 (8): 3216-3229Li Y, et al. (2000) J Biol Chem ; 275 (47): 37187-37193Kinley AW, et al. (2003) Curr Biol ; 13 (5): 384-393

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-489

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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