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Western blot analysis of COTL1 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of COTL1 using rabbit polyclonal COTL1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COTL1 gene.
Western blot analysis of COTL1 Antibody in Hela cell line lysates (35ug/lane)

COTL1 Antibody

CATALOG NUMBER: 63-657

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This COTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-142 amino acids from the C-terminal region of human COTL1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COTL1
Additional Names:
Coactosin-like protein, COTL1, CLP
Protein Accession Number:
Q14019
Protein Gi Number:
21759076
Ncbi Gene Id Number:
23406
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
Background Reference 1:
Provost,P., et.al., Proc. Natl. Acad. Sci. U.S.A. 96 (5), 1881-1885 (1999)
Background Reference 2:
Provost,P., et.al., J. Biol. Chem. 276 (19), 16520-16527 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-657

List Size:
400 ul

List Price:

$495.00

Datasheet
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