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45-124 (2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-124 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-124 (0.5ug/ml) staining of Mouse Kidney (A) and Skeletal Muscle (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-124 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and vesicle staining. The nuclear stain is
45-124 (2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

COX-1 Antibody

CATALOG NUMBER: 45-124

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: C-QDDGPAVERPSTEL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and 69.0kDa according to Mouse NP_032995.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus and vesicles of NIH3T3 cells. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTGS1
Additional Names:
PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Protein Accession Number:
NP_000953.2,NP_542158.1
Protein Gi Number:
18104967
Ncbi Gene Id Number:
5742
Background Reference 1:
Yokoyama C, Tanabe T. Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme. Biochem Biophys Res Commun. 1989 Dec 15;165(2):888-94.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-124

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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