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Western blot analysis in CEM cell line lysates (35ug/lane).
COX10 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

COX10 Antibody

CATALOG NUMBER: 55-363

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This COX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-410 amino acids from the C-terminal region of human COX10.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COX10
Additional Names:
Protoheme IX farnesyltransferase, mitochondrial, 251-, Heme O synthase, COX10
Protein Accession Number:
Q12887
Protein Gi Number:
292495084
Ncbi Gene Id Number:
1352
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytochrome c oxidase (COX), the terminal component of themitochondrial respiratory chain, catalyzes the electron transferfrom reduced cytochrome c to oxygen. This component is aheteromeric complex consisting of 3 catalytic subunits encoded bymitochondrial genes and multiple structural subunits encoded bynuclear genes. The mitochondrially-encoded subunits function inelectron transfer, and the nuclear-encoded subunits may function inthe regulation and assembly of the complex. This nuclear geneencodes heme A:farnesyltransferase, which is not a structuralsubunit but required for the expression of functional COX andfunctions in the maturation of the heme A prosthetic group of COX.This protein is predicted to contain 7-9 transmembrane domainslocalized in the mitochondrial inner membrane. A gene mutation,which results in the substitution of a lysine for an asparagine(N204K), is identified to be responsible for cytochrome c oxidasedeficiency. In addition, this gene is disrupted in patients withCMT1A (Charcot-Marie-Tooth type 1A) duplication and with HNPP(hereditary neuropathy with liability to pressure palsies)deletion.
Background Reference 1:
Chen, Z., et al. Oncogene 29(30):4362-4368(2010)
Background Reference 2:
Vitali, M., et al.Neural Transm 116(12):1635-1641(2009)
Background Reference 3:
Dassa, E.P., et al. EMBO Mol Med 1(1):30-36(2009)
Background Reference 4:
Veluthakal, R., et al. Diabetes 56(1):204-210(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-363

List Size:
400 ul

List Price:

$495.00

Datasheet
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