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Western blot analysis of extracts of various cell lines, using Cox2 antibody (14-486) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human liver cancer using Cox2 antibody (14-486) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using Cox2 antibody (14-486) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using Cox2 antibody (14-486) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Cox2 antibody (14-486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Cox2 Antibody

CATALOG NUMBER: 14-486

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
A-549
Positive Control 2:
HeLa
Positive Control 3:
Mouse spleen
Positive Control 4:
Mouse lung
Positive Control 5:
Rat lung
Positive Control 6:
Rat kidney
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PTGS2
Additional Names:
COX-2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, prostaglandin G/H synthase and cyclooxygenase
Ncbi Gene Id Number:
5743
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-486

List Size:
100 uL

List Price:

$436.00

Datasheet
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