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45-424 (0.01ug/ml) staining of human muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-424 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-424 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-424 (0.01ug/ml) staining of human muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

COX4I1 Antibody

CATALOG NUMBER: 45-424

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QGLASKWDYEKNE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 18kDa band observed in human skeletal muscle lysates (calculated MW of 19.6kDa according to NP_001852.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Thyroid and Testis. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms 1 and 4 (NP_001852.1; NP_001305726.1). Reported variants represent identical protein: NP_001305715.1, NP_001852.1. This product may cross-react to COX4I2 (GeneID 84701).
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
COX4I1
Additional Names:
COX4I1, cytochrome c oxidase subunit IV isoform 1 , COX4, COXIV, MGC72016
Protein Accession Number:
NP_001852.1,NP_001305726.1
Protein Gi Number:
4502981
Ncbi Gene Id Number:
1327;84701
Background Reference 1:
Fukuda R, Zhang H, Kim JW, Shimoda L, Dang CV, Semenza GL. HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells. Cell. 2007 Apr 6;129(1):111-22.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-424

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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