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Western blot analysis of extracts of various cell lines, using COX6A1 antibody (19-164) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using COX6A1 antibody (19-164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

COX6A1 Antibody

CATALOG NUMBER: 19-164

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:20 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
MCF7
Positive Control 3:
Mouse kidney
Positive Control 4:
Mouse heart
Positive Control 5:
Mouse liver
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 12kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
COX6A1
Additional Names:
Cytochrome c oxidase subunit 6A1, mitochondrial, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver, COX VIa-L, COX6A1, COX6AL
Ncbi Gene Id Number:
1337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-164

List Size:
100 uL

List Price:

$436.00

Datasheet
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