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Western blot analysis in ZR-75-1,A549,NCI-H292 cell line lysates (35ug/lane).
COX6B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1,A549,NCI-H292 cell line lysates (35ug/lane).

COX6B1 Antibody

CATALOG NUMBER: 56-356

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This COX6B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from the C-terminal region of human COX6B1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
10 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COX6B1
Additional Names:
Cytochrome c oxidase subunit 6B1, Cytochrome c oxidase subunit VIb isoform 1, COX VIb-1, COX6B1, COX6B
Protein Accession Number:
P14854
Protein Gi Number:
117115
Ncbi Gene Id Number:
1340
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytochrome c oxidase (COX), the terminal enzyme of themitochondrial respiratory chain, catalyzes the electron transferfrom reduced cytochrome c to oxygen. It is a heteromeric complexconsisting of 3 catalytic subunits encoded by mitochondrial genesand multiple structural subunits encoded by nuclear genes. Themitochondrially-encoded subunits function in electron transfer, andthe nuclear-encoded subunits may be involved in the regulation andassembly of the complex. This nuclear gene encodes subunit VIb.Mutations in this gene are associated with severe infantileencephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 andCOX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2,respectively.
Background Reference 1:
Lazarou, M., et al. FEBS J. 276(22):6701-6713(2009)
Background Reference 2:
Massa, V., et al. Am. J. Hum. Genet. 82(6):1281-1289(2008)
Background Reference 3:
Sirchia, R., et al. Biol. Chem. 388(5):457-465(2007)
Background Reference 4:
Schmidt, T.R., et al. Gene 286(1):13-19(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-356

List Size:
400 ul

List Price:

$495.00

Datasheet
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