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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
COX7A2L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

COX7A2L Antibody

CATALOG NUMBER: 56-357

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This COX7A2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the Central region of human COX7A2L.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
13 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COX7A2L
Additional Names:
Cytochrome c oxidase subunit 7A-related protein, mitochondrial, COX7a-related protein, Cytochrome c oxidase subunit VIIa-related protein, EB1, COX7A2L, COX7AR, COX7RP
Protein Accession Number:
O14548
Ncbi Gene Id Number:
9167
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytochrome c oxidase (COX), the terminal component of themitochondrial respiratory chain, catalyzes the electron transferfrom reduced cytochrome c to oxygen. This component is aheteromeric complex consisting of 3 catalytic subunits encoded bymitochondrial genes and multiple structural subunits encoded bynuclear genes. The mitochondrially-encoded subunits function inelectron transfer, and the nuclear-encoded subunits may function inthe regulation and assembly of the complex. This nuclear geneencodes a protein similar to polypeptides 1 and 2 of subunit VIIain the C-terminal region, and also highly similar to the mouseSig81 protein sequence. This gene is expressed in all tissues, andupregulated in a breast cancer cell line after estrogen treatment.It is possible that this gene represents a regulatory subunit ofCOX and mediates the higher level of energy production in targetcells by estrogen.
Background Reference 1:
Fornuskova, D., et al. Biochem. J. 428(3):363-374(2010)
Background Reference 2:
Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
Background Reference 3:
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Background Reference 4:
Schmidt, T.R., et al. J. Mol. Evol. 57(2):222-228(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-357

List Size:
400 ul

List Price:

$495.00

Datasheet
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