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43-253 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-253 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CP110 Antibody

CATALOG NUMBER: 43-253

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CEKSLARIQEAS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Testis lysates (calculated MW of 113kDa according to NP_001185951.1). Recommended concentration: 0.3-1ug/ml.Additional bands of unknown identity were also consistently observed at 65kDa and 37kDa. These bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/specific breakdown fragments been reported?
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001185951.1; NP_055526.3).
Predicted Molecular Weight:
Approx 110kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CP110
Additional Names:
CP110, -, Cep110, DKFZp781G1416, KIAA0419, OTTHUMP00000162162, centrosomal protein CP110, centrosomal protein of 110 kDa
Protein Accession Number:
NP_001185951.1,NP_055526.3
Protein Gi Number:
312222737
Ncbi Gene Id Number:
9738
Background Reference 1:
D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, Washburn MP, Dynlacht B, Pagano M.SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-253

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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