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Western blot analysis of CPN1 using rabbit polyclonal CPN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.
CPN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CPN1 using rabbit polyclonal CPN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.

CPN1 Antibody

CATALOG NUMBER: 56-714

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-240 amino acids from the Central region of human CPN1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPN1
Additional Names:
Carboxypeptidase N catalytic chain, CPN, Anaphylatoxin inactivator, Arginine carboxypeptidase, Carboxypeptidase N polypeptide 1, Carboxypeptidase N small subunit, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, Serum carboxypeptidase N, SCPN, CPN1, ACBP
Protein Accession Number:
P15169
Protein Gi Number:
115896
Ncbi Gene Id Number:
1369
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Carboxypeptidase N is a plasma metallo-protease thatcleaves basic amino acids from the C terminal of peptides andproteins. The enzyme is important in the regulation of peptideslike kinins and anaphylatoxins, and has also been known askininase-1 and anaphylatoxin inactivator. This enzyme is a tetramercomprised of two identical regulatory subunits and two identicalcatalytic subunits; this gene encodes the catalytic subunit.Mutations in this gene can be associated with angioedema or chronicurticaria resulting from carboxypeptidase N deficiency. [providedby RefSeq].
Background Reference 1:
Speliotes, E.K., et al. Hepatology 52(3):904-912(2010)
Background Reference 2:
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Background Reference 3:
Du, X.Y., et al. J. Biol. Chem. 284(2):751-758(2009)
Background Reference 4:
Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-714

List Size:
400 ul

List Price:

$495.00

Datasheet
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