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Western Blot at 1:1000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CPN1 antibody in K562, CEM, HEpG2 cell line lysates and mouse liver tissue lysates (35ug/lane)
Western blot analysis of CPN1 using rabbit polyclonal CPN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.
Western Blot at 1:1000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

CPN1 Antibody

CATALOG NUMBER: 62-834

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human CPN1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPN1
Additional Names:
Carboxypeptidase N catalytic chain, CPN, Anaphylatoxin inactivator, Arginine carboxypeptidase, Carboxypeptidase N polypeptide 1, Carboxypeptidase N small subunit, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, Serum carboxypeptidase N, SCPN, CPN1, ACBP
Protein Accession Number:
P15169
Protein Gi Number:
115896
Ncbi Gene Id Number:
1369
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CPN1 is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The protein is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This protein is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits.
Background Reference 1:
Davis,D.A., Singer,K.E. Blood 105 (12), 4561-4568 (2005)
Background Reference 2:
Riley,D.A., Tan,F. Genomics 50 (1), 105-108 (1998)
Background Reference 3:
Hendriks,D., Vingron,M. Biol. Chem. Hoppe-Seyler 374 (9), 843-849 (1993)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-834

List Size:
400 ul

List Price:

$495.00

Datasheet
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