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Western blot analysis of CPN2 antibody in jurkat cell line lysates (35ug/lane)
CPN2 Antibody (RB18703) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CPN2 antibody in jurkat cell line lysates (35ug/lane)

CPN2 Antibody

CATALOG NUMBER: 62-846

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CPN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the N-terminal region of human CPN2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPN2
Additional Names:
Carboxypeptidase N subunit 2, Carboxypeptidase N 83 kDa chain, Carboxypeptidase N large subunit, Carboxypeptidase N polypeptide 2, Carboxypeptidase N regulatory subunit, CPN2, ACBP
Protein Accession Number:
P22792
Protein Gi Number:
334302917
Ncbi Gene Id Number:
1370
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CPN2, the 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Background Reference 1:
Liu,T., Qian,W.J. J. Proteome Res.(6), 2070-2080 (2005)
Background Reference 2:
Riley,D.A., Tan,F. Genomics 50 (1), 105-108 (1998)
Background Reference 3:
Skidgel,R.A. Biochem. Biophys. Res. Commun. 154 (3), 1323-1329 (1988)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-846

List Size:
400 ul

List Price:

$495.00

Datasheet
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