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Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

CPSF6 Antibody

CATALOG NUMBER: 60-961

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Mouse, Xenopus
Immunogen:
This CPSF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human CPSF6.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPSF6
Additional Names:
Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, CFIm68, CPSF 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF6, CFIM68
Protein Accession Number:
Q16630
Protein Gi Number:
88909266
Ncbi Gene Id Number:
11052
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one subunit of acleavage factor required for 3' RNA cleavage and polyadenylationprocessing. The interaction of the protein with the RNA is one ofthe earliest steps in the assembly of the 3' end processing complexand facilitates the recruitment of other processing factors. Thecleavage factor complex is composed of four polypeptides. This geneencodes the 68kD subunit. It has a domain organization reminiscentof spliceosomal proteins.
Background Reference 1:
Lee, K., et al. Cell Host Microbe 7(3):221-233(2010)
Background Reference 2:
Ruepp, M.D., et al. Mol. Biol. Cell 20(24):5211-5223(2009)
Background Reference 3:
Xin, X., et al. Genome Res. 19(7):1262-1269(2009)
Background Reference 4:
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-961

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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