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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

CPSF7 Antibody

CATALOG NUMBER: 56-247

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CPSF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 427-455 amino acids from the C-terminal region of human CPSF7.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPSF7
Additional Names:
Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59, CPSF 59 kDa subunit, Pre-mRNA cleavage factor Im 59 kDa subunit, CPSF7
Protein Accession Number:
Q8N684
Protein Gi Number:
74759932
Ncbi Gene Id Number:
79869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CPSF7 is probable a component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
Background Reference 1:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 2:
Lim, J., et al. Cell 125(4):801-814(2006)
Background Reference 3:
Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004)
Background Reference 4:
Zhou, Z., et al. Nature 419(6903):182-185(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-247

List Size:
400 ul

List Price:

$495.00

Datasheet
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