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CPT1A Antibody

CATALOG NUMBER: 46-959

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-PETDSHRFGKHLRQ
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave next to a 90kDa band additional bands at approx. 37kDa and 25kDa in Mouse and Rat Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 88.3kDa according to NP_038523.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cpt1a
Additional Names:
Cpt1a, carnitine palmitoyltransferase 1A, liver, CPT1, CPT1-L, L-CPT1, C730027G07, carnitine palmitoyltransferase 1A, carnitine palmitoyltransferase 1, liver
Protein Accession Number:
NP_038523.2
Protein Gi Number:
162287142
Ncbi Gene Id Number:
12894(mouse);25757(rat);
Background Reference 1:
Mazzarelli P, Pucci S, Bonanno E, Sesti F, Calvani M, Spagnoli LG. Carnitine palmitoyltransferase I in human carcinomas: a novel role in histone deacetylation? Cancer Biol Ther. 2007 Oct;6(10):1606-13.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-959

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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