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Western blot analysis of anti-CPT1A Pab in Y79 cell line lysate (35ug/lane).
Left image is paraformaldehyde-fixed and paraffin-embedded cow lactating with CPT1A Pab , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining, right image is contrast, did not add the antibody. This data was kindly offered by Hideaki Hayashi, University of Bern, Switzerland.
Western blot analysis of anti-CPT1A Pab in Y79 cell line lysate (35ug/lane).

CPT1A Antibody

CATALOG NUMBER: 61-413

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Human
Immunogen:
This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-636 amino acids from the C-terminal region of human CPT1A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPT1A
Additional Names:
Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A, CPT1A, CPT1
Protein Accession Number:
P50416
Protein Gi Number:
56405343
Ncbi Gene Id Number:
1374
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.
Background Reference 1:
Rasmussen, B.B., et al., J. Clin. Invest. 110(11):1687-1693 (2002).
Background Reference 2:
Ogawa, E., et al., J. Hum. Genet. 47(7):342-347 (2002).
Background Reference 3:
Cook, G.A., et al., Am. J. Med. Sci. 318(1):43-48 (1999).
Background Reference 4:
IJlst, L., et al., J. Clin. Invest. 102(3):527-531 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-413

List Size:
400 ul

List Price:

$495.00

Datasheet
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