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45-427 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-427 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CPT1B Antibody

CATALOG NUMBER: 45-427

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DIADLFQVPKAYS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Heart shows staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognise human isoforms a (NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1), isoform c (NP_001138606.1) and isoform d (NP_001138608.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CPT1B
Additional Names:
CPT1B, carnitine palmitoyltransferase I, muscle, carnitine palmitoyltransferase 1B (muscle) , CPT1-M, M-CPT1, KIAA1670, CPTI, muscle, carnitine O-palmitoyltransferase I, mitochondrial muscle isoform, carnitine palmitoyltransferase 1A (muscle), carnitine palmitoyltransferase 1B, carnitine palmitoyltransferase 1B (muscle), CPT1M, FLJ55729, FLJ58750, MCCPT1, MCPT1, carnitine palmitoyltransferase I-like
Protein Accession Number:
NP_689452.1,NP_689451.1,NP_004368.1,NP_001138609.1,NP_001138607.1,NP_001138606.1,NP_001138608.1
Protein Gi Number:
4758050
Ncbi Gene Id Number:
1375
Background Reference 1:
Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I. Biochim Biophys Acta. 1996 Jun 7;1307(2):157-61.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-427

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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