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Western blot analysis of CPT1B using rabbit polyclonal CPT1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of CPT1B using rabbit polyclonal CPT1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).

CPT1B Antibody

CATALOG NUMBER: 61-417

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CPT1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-637 amino acids from the C-terminal region of human CPT1B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CPT1B
Additional Names:
Carnitine O-palmitoyltransferase 1, muscle isoform, CPT1-M, Carnitine O-palmitoyltransferase I, muscle isoform, CPT I, CPTI-M, Carnitine palmitoyltransferase 1B, Carnitine palmitoyltransferase I-like protein, CPT1B, KIAA1670
Protein Accession Number:
Q92523
Protein Gi Number:
2493497
Ncbi Gene Id Number:
1375
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CPT1B is a member of the carnitine/choline acetyltransferase family, and is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria.
Background Reference 1:
Dai, J., et al., Biochem. Biophys. Res. Commun. 301(3):758-763 (2003).
Background Reference 2:
van der Leij, F.R., et al., J. Biol. Chem. 277(30):26994-27005 (2002).
Background Reference 3:
Hirosawa, M., et al., DNA Res. 8(1):1-9 (2001).
Background Reference 4:
Yamazaki, N., et al., J. Biol. Chem. 275(41):31739-31746 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-417

List Size:
400 ul

List Price:

$495.00

Datasheet
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