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Western blot analysis of extracts of various cell lines, using CRABP2 antibody (22-012) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CRABP2 antibody (22-012) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CRABP2 antibody (22-012). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CRABP2 antibody (22-012) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.

CRABP2 Antibody

CATALOG NUMBER: 22-012

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-94 of human CRABP2 (NP_001869.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
SW480
Positive Control 3:
A-431
Positive Control 4:
22Rv1
Positive Control 5:
HT-29
Positive Control 6:
293T
Predicted Molecular Weight:
Observed: 15kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CRABP2
Additional Names:
CRABP2, CRABP-II, RBP6
Ncbi Gene Id Number:
1382
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-012

List Size:
100 uL

List Price:

$436.00

Datasheet
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