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<strong>Figure 1 Immunofluorescence Validation of CRBN in HeLa Cells</strong><br>Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with 8115 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
<strong>Figure 2 WB Validation in Human Testis </strong><br>Loading: 15 μg of human testis lysate Antibodies: CRBN 8115, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 0.5 μg/mLLane B: 1 μg/mL
<strong>Figure 3 WB Validation in Human Cells </strong><br>Loading: 15 μg of cell lysate Antibodies: CRBN 8115, 1 μg/mL,1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of mouse tissue lysate Antibodies: CRBN 8115, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of rat tissue lysate Antibodies: CRBN 8115, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 Immunofluorescence Validation of CRBN in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with 8115 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Trial Size

CRBN Antibody

CATALOG NUMBER: 8115

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.
The immunogen is located within the last 50 amino acids of CRBN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 49 kDa
Observed: 54 kDa
Purification:
CRBN antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CRBN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CRBN antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CRBN
Additional Names:
Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A
Protein Accession Number:
NP_057386
Protein Gi Number:
39545580
Ncbi Gene Id Number:
51185
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).
Background Reference 1:
Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6.
Background Reference 2:
Gandhi AK, Kang J, Havens CG, et al. Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4(CRBN). Br. J. Haematol. 2014; 164:811-21.
Background Reference 3:
Liu J, Ye J, Zou X, et al. CRL4A (CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat. Commun. 2014; 5:3924.
Background Reference 4:
Heintel D, Rocci A, Ludwig H, et al. High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone. Br. J. Haematol. 2013; 161:695-700.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8115

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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