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43-265 (1ug/ml) staining of Mouse (A) and Rat (B) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-265 (1ug/ml) staining of Mouse (A) and Rat (B) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

creatine kinase M-type Antibody

CATALOG NUMBER: 43-265

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QKIEEIFKKAGHP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in Mouse and Rat Heart lysates (calculated MW of 43kDa according to Mouse NP_031736.1 and Rat NP_036662.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Heart for this particular batch.
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
creatine kinase M-type
Additional Names:
CKM, creatine kinase, muscle, CKMM, M-CK, creatine kinase M chain, creatine kinase M-type, creatine kinase-M
Protein Accession Number:
NP_001815.2
Protein Gi Number:
21536288
Ncbi Gene Id Number:
1158
Background Reference 1:
Diguet N, Mallat Y, Ladouce R, Clodic G, Prola A, Tritsch E, Blanc J, Larcher JC, Delcayre C, Samuel JL, Friguet B, Bolbach G, Li Z, Mericskay M.Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-265

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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