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Western blot analysis of lysed extracts from 293, HT29 and HeLa cells using CREB (Ab-133).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Ab-133).
Western blot analysis of lysed extracts from 293, HT29 and HeLa cells using CREB (Ab-133).

CREB (Ab 133) Antibody

CATALOG NUMBER: 79-293

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CREB (Ab-133) antibody was raised against a peptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.
Predicted Molecular Weight:
43 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CREB1
Additional Names:
CREB, NR3C3, CREB-1, CREB1
Protein Accession Number:
NP_004370.1
Protein Gi Number:
4758054
Ncbi Gene Id Number:
1385
Background:
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Background Reference 1:
Xing J, et al. (1998) Mol Cell Biol 18 (4): 1946-55. Tan Y, et al. ( 1996) EMBO J; 15 (17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27): 25184-9. Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-293

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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