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Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase (lamda-Ptase, 1200 units for 30 min).
Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase (lamda-Ptase, 1200 units for 30 min).

CREB (phospho Ser133) Antibody

CATALOG NUMBER: 50-221

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Dog, Human, Mouse, Rat, Sheep, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Dog, Human, Mouse, Rat, Sheep, Zebrafish
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Predicted Molecular Weight:
45
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
CREB antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Creb1
Protein Accession Number:
P15337
Protein Gi Number:
117435
Ncbi Gene Id Number:
81646
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogenactivated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
Background Reference 1:
Johannessen M, Delghandi MP, Moens U (2004) What turns CREB on? Cellular Signalling 16:1211-1227.
Background Reference 2:
Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr Biol 5:747-757.
Background Reference 3:
Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34:221-233.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-221

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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