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43-372 (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-372 (5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-372 (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CREB3 Antibody

CATALOG NUMBER: 43-372

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HHDHTYSLPRET
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in lysates of cell line HepG2 (calculated MW of 41.4kDa according to NP_006359.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CREB3
Additional Names:
CREB3, cAMP responsive element binding protein 3, LUMAN, LZIP, CREB-3, basic leucine zipper protein, cAMP-responsive element-binding protein 3, cyclic AMP response element (CRE)-binding protein/activating transcription factor 1, cyclic AMP-responsive element-binding protein 3, leucin zipper proitein, transcription factor LZIP-alpha
Protein Accession Number:
NP_006359.3
Protein Gi Number:
22219462
Ncbi Gene Id Number:
10488
Background Reference 1:
Kim HC, Choi KC, Choi HK, Kang HB, Kim MJ, Lee YH, Lee OH, Lee J, Kim YJ, Jun W, Jeong JW, Yoon HG.HDAC3 selectively represses CREB3-mediated transcription and migration of metastatic breast cancer cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-372

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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